Table 1 Characteristics of primers used for detection of mollicutes investigated in this study
From: Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor
| Mycoplasma species | Primer designation | Target gene | Gene locus | Product | Amplicon size (bp) | Sequence (5′-3′) | References |
|---|---|---|---|---|---|---|---|
| M. hominis |
Mhp120’F Mhp120’R | p120’ | MHO_RS02125 | DUF885 family protein | 190 |
GTTTTAGAACGTAAAATTCTA CATCAGCAGGATTGTTTAAAGCT | This studya |
|
Mh16SF Mh16SR | rrnA16S | MHO_RS01540 | 16S ribosomal RNA | 164 |
GGTAATGGCCCACCAAGACTAT GAAGACCTTCATCGTGCACGCTG | This studya | |
| M. genitalium |
MgPa335F MgPa432R | mgpA | MG_RS01075 | Adhesin protein | 78 |
GAGAAATACCTTGATGGTCAGCAA GTTAATATCATATAAAGCTCTACCGTTGTTATC | [43] |
|
Mg16SF MG16SR | rrnA16S | MG_RS00775 | 16S ribosomal RNA | 195 |
TAGCTAATACCGCATAAGAACTTT CTCCCGTAGGAGTATGGGCCGTGT | This studya | |
| M. fermentans |
RW 005 RW 004 | IS1630I | MBIO_RS03870 | IS30-like element | 205 |
GGTTATTCGATTTCTAAATCGCCT GGACTATTGTCTAAACAATTTCCC | [44] |
|
Mf16SF Mf16SR | rrnA16S | MBIO_RS4090 | 16S ribosomal RNA | 186 |
TTGACGGTACCTTATTAGAAA TCTACACAACTCTAGCCTG | This studya | |
| U. urealyticum |
UUureF UUureR | ureB | UUR8_RS02385 | Urease subunit beta | 100 |
GATCACATTTCCACTTATTTGAAACA AAACGACGTCCATAAGCAACTTTA | [45] |
|
Uu16SF Uu16SR | rrnA16S | UUR8_RS00640 | 16S ribosomal RNA | 181 |
TGCCTGGGTAGTACATTCGCAAGAA GACAACCATGCACCACCTGTC | This studya | |
| U. parvum |
UPureF UPureF | ureB | UPA3_RS02275 | Urease subunit beta | 99 |
GATCACATTTTCACTTGTTTG AAGTG AACGTCGTCCATAAGCAACTTTG | [45] |
|
Up16SF Up16SF | rrnA16S | UPA3_RS00595 | 16S ribosomal RNA | 183 |
CTTTTATATGGGAAGAAACGCT GCATCTAGATTTAATACC | This studya | |
| Human DNA |
HmitoDNAF HmitoDNAR | MT-CO3 | YP_003024032.1 | Cytochrome c oxidase subunit III | 208 |
GGATAATCCTATTTATTACCTCAGAAG AGACTATGGTGAGCTCAGGTGATTGAT | This studya |