Table 1 PCR primer sequences used to amplify HHV8 latent and lytic mRNAs
From: Effect of electrochemotherapy on human herpesvirus 8 kinetics in classic Kaposi sarcoma
| Locus | Primer name | Sequences (5′ – 3′) | cDNA Amplicon (bp) |
|---|---|---|---|
| Human | |||
| Il-1α |
DM151 DM152 | GTCTCTGAATCAGAAATCCTTCTATC CATGTCAAATTTCACTGCTTCATCC | 421 |
| HHV8 latent | |||
| ORF72 |
ORF72A ORF72B | CCGCGCTTTTTAACTTCTGACTCT GCTGATAATAGAGGCGGGCAATGA | 507 |
| ORF73 |
ORF73A ORF73B |
ACTATGGAAGATTGTAGG TATATGTGTATTGTCAGAAC | 106 |
| HHV8 lytic | |||
| K2 |
ORFK2F ORFK2R |
ATTGAGTCTCTGAATGAG TTCAAGTTGTGGTCTATC | 155 |
| K8 |
ORFK8F ORFK8R |
AAAGCATACACAAGACAG AAATAATCTGTTCCTTATGTG | 100 |
| K8.1 |
ORFK8.1F ORFK8.1R |
CCGATGCCTTAATATCAG TTCCTCTAGTCGTTGTAG | 155 |
| K10.5/K10.6 |
VIRF3F VIRF3R |
GAGTTTTCACCCACAAATG CAGGACTCACCTACACAG | 107 |
| K10/K10.1 |
VIRF4F VIRF4R |
TGTTCAGTCGTTATATCA TATTCAGTGTCTGTTGTC | 182 |
| ORF16 |
ORF16F ORF16R |
TGGCTATACTGACCTTTG GCTTCATACGCATATACAG | 78 °C |